Isolation of primary murine CD4+ T cells and generation of T helper cell subsets in vitro
Oestreich Lab Primary T Cell Isolation and Culture – PDF
Oestreich Lab T helper Subset Polarizing Conditions– PDF
Co-Immunoprecipitation (Co-IP)
Oestreich Lab Co-IP Protocol – PDF
Chromatin immunoprecipitation (ChIP)
Oestreich Lab ChIP Crosslinking Protocol – PDF
Oestreich Lab ChIP Protocol (after Crosslinking) – PDF